Ecotourism is able to contribute to biodiversity and to ensure the ecosystem function in Latvia through a complicated process of fulfilment of the established criteria for a valid ecotourism. The tourism flow in protected nature territories should be monitored, and the appropriate environmental management, education and a code of conduct need to be introduced. Ecotourism will serve for reducing the social and economic pressures on biodiversity and will promote responsible actions in support of environmentally friendly technologies and approaches. The areas selected for this study are the Natura 2000 territories that have undertaken the development of ecotourism facilities.