Human thermal comfort depends on combinations of clothing, climate and physical activity. The thermal exchange between body and environment takes place through heat conduction, convection and radiation, but the exchange of body moisture takes place through perspiration and sweating. Heat and moisture transport processes are influenced both in the form of diffusion, and by ventilation through the garment fabric caused by the movement of human in the environment.To verify microclimate regulatory clothes prototype with electrical cooling system compliance with the general requirements to the garment, it is scheduled to assess the physiologically - hygienic point of wearing by the appropriate operating conditions, during activities by measuring temperature changes in different locations in space between the body and clothes.The analysis of human microclimate temperature modeling experimental results reveals the negative impact of non-evaporative materials on the possibility of vapour removal through the garment surface, which causes additional temperature rise pointing to the importance of this factor in ensuring the thermal comfort.