Model-based fed-batch cultivation approach of non-recombinant Escherichia coli bacteria are presented. Aim of current research was to utilize mathematical modeling of fermentation process to precisely calculate exponential feeding rates for succesive biomass growth processes. In this case were used models which state mass balance for biomass (X), substrate (S), acetate (A), vessel volume (V) and additional model for bioreactor mass flows (F). In such a way we achieved controlled and predefined biomass growth according to the process model. Using well defined fed-batch media which consisted from salts and glucose, in most successive runs were achieved E. coli cultures with biomass concentrations up to 60 g/l.