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Publikācija: The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg

Publication Type Full-text conference paper published in conference proceedings indexed in SCOPUS or WOS database
Funding for basic activity Unknown
Defending: ,
Publication language English (en)
Title in original language The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg
Field of research 1. Natural sciences
Sub-field of research 1.4 Chemical sciences
Authors Kristīne Veģere
Ieva Bērza
Simona Larsona
Andris Dišlers
Juris Vanags
Keywords Recombinant Hepatitis B core-antigen, virus like particles, T5 promoter, expression
Abstract Biotechnological aspects in the field of recombinant protein production are analysed in this work, which includes the investigation of factors for the production of virus like particles (VLPs) formed by recombinant Hepatitis B virus core-antigen (HBcAg) in inducible bacterial (E. coli) expression system as well as purification of these particles by gelfiltration and ion-exchange chromatography. Biomass yield and VLP production level depended on the cultivation conditions such carbon source and temperature. As inductor for protein expression lactose was used.
DOI: 10.1007/978-3-642-34197-7_28
Hyperlink: http://link.springer.com/chapter/10.1007/978-3-642-34197-7_28 
Reference Ruģele, K., Bērza, I., Larsona, S., Dišlers, A., Vanags, J. The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg. In: IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics, Latvia, Rīga, 10-12 October, 2012. Berlin: Springer Berlin Heidelberg, 2013, pp.108-111. ISBN 978-3-642-34196-0. e-ISBN 978-3-642-34197-7. ISSN 1680-0737. e-ISSN 1433-9277. Available from: doi:10.1007/978-3-642-34197-7_28
Additional information Citation count:
  • Scopus  0
ID 14434