RTU Research Information System
Publikācija: The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg
| Publication Type | Full-text conference paper published in conference proceedings indexed in SCOPUS or WOS database |
|---|---|
| Funding for basic activity | Unknown |
| Defending: | , |
| Publication language | English (en) |
| Title in original language | The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg |
| Field of research | 1. Natural sciences |
| Sub-field of research | 1.4 Chemical sciences |
| Authors |
Kristīne Veģere
Ieva Bērza Simona Larsona Andris Dišlers Juris Vanags |
| Keywords | Recombinant Hepatitis B core-antigen, virus like particles, T5 promoter, expression |
| Abstract | Biotechnological aspects in the field of recombinant protein production are analysed in this work, which includes the investigation of factors for the production of virus like particles (VLPs) formed by recombinant Hepatitis B virus core-antigen (HBcAg) in inducible bacterial (E. coli) expression system as well as purification of these particles by gelfiltration and ion-exchange chromatography. Biomass yield and VLP production level depended on the cultivation conditions such carbon source and temperature. As inductor for protein expression lactose was used. |
| DOI: | 10.1007/978-3-642-34197-7_28 |
| Hyperlink: | http://link.springer.com/chapter/10.1007/978-3-642-34197-7_28  |
| Reference | Ruģele, K., Bērza, I., Larsona, S., Dišlers, A., Vanags, J. The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg. In: IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics, Latvia, Rīga, 10-12 October, 2012. Berlin: Springer Berlin Heidelberg, 2013, pp.108-111. ISBN 978-3-642-34196-0. e-ISBN 978-3-642-34197-7. ISSN 1680-0737. e-ISSN 1433-9277. Available from: doi:10.1007/978-3-642-34197-7_28 |
| Additional information |
Citation count:
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| ID | 14434 |
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