Polyisoprene/Nanostructured carbon (PNC) composites have drawn their attention due to their pronounced piezoresistive properties which can be used for stress/strain sensor applications [1, 2]. To achieve optimal piezoresistive properties of PNC composite samples it is crucial to identify all the sensor-effect influencing factors during sample manufacturing process. It has already been shown that conductive nanoparticle network in the PNC composite largely forms during vulcanization process [3]. Our current work was done to find out an influence of two roll mill (TRM) mixing of a raw PNC composite on AC conductance of finalized (vulcanized) PNC composite samples.