The properties of the electrospun nanofibers ensure their usability for nanobiocomposites and medical applications [1], therefore acquisition methods that would be effective and successfully integrated not only in laboratories, but also in the production are investigated. Sodium chlorophyllin (analogues of food additive E 141 according to the Codex Alimentarius [2]) is a component of neutralized extractive substances derived from spruce foliage. Sodium chlorophyllin contains derivatives of "a" and "b" chlorophyll (chlorines, sodium salts of chlorophylline acids etc.), sodium salts of resinous acids (pimaric-, isopimaric-, abietic- and labdane types), sodium salts of fatty acids (mainly oleic-, stearic- and linoleic). Sodium chlorophyllin is used as active food additive and color, as well as in cosmetics (creams, deodorants etc.). The product shows bacteriostatic, regenerative and deodorant properties [3]. The effect of sodium chlorophyllin concentration (С32H2CN4N) on polyvinyl alcohol (PVA) electrospun nanofiber web are discussed and interpreted by an attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, atomic force microscopy (AFM), mechanical testing and microbiological test.