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Publikācija: Application of Conventional HPLC RI Technique for Sugar Analysis in Hydrolysed Hay

Publication Type Scientific article indexed in SCOPUS or WOS database
Funding for basic activity Unknown
Defending: ,
Publication language English (en)
Title in original language Application of Conventional HPLC RI Technique for Sugar Analysis in Hydrolysed Hay
Field of research 2. Engineering and technology
Sub-field of research 2.7 Environmental engineering and energetics
Authors Kristīna Tihomirova
Brigita Daļecka
Linda Mežule
Keywords Lignocellulosic biomass, sugar determination, DNS method, HPLC RI
Abstract To determine the potential biofuel yield and necessary technological parameters a known concentration and type of fermentable sugars should be produced during chemical or biological extraction from lignocellulose. The most popular method for sugar interpretation and quantification is liquid chromatography (HPLC) using refractive index (RI) detector. The aim of this research was to show the applicability of the high–performance liquid chromatography using refractive index (HPLC RI) technique for sugar interpretation in hydrolysed hay and possible solutions for optimisation of this method. Analysis of hydrolysed hay with standard additive showed low recovery of sugar concentrations and inconsistencies with dinitrosalicylic acid (DNS) method, which was mostly due to low separation of peaks of these sugars on the chromatograms. As result HPLC RI method was useful for qualitative analysis of sugars only, not for its quantification.
Hyperlink: http://agronomy.emu.ee/wp-content/uploads/2016/05/Vol14_nr5_Tihomirova.pdf#abstract-3949 
Reference Tihomirova, K., Daļecka, B., Mežule, L. Application of Conventional HPLC RI Technique for Sugar Analysis in Hydrolysed Hay. Agronomy Research, 2016, Vol.14, No.5, pp.1713-1719. ISSN 1406-894X.
Additional information Citation count:
  • Scopus  0
ID 23168