In Vitro Characterization Perspectives Using Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR PAS) and Diffuse Reflectance Infrared Spectroscopy (DRIFT)

Key Engineering Materials 2017
Agnese Brangule, Ingus Skadiņš, Aigars Reinis, Kārlis-Agris Gross, Juta Kroiča

The activity of antibacterial material is conventionally estimated by using an indirect method – a bacteria suspension is inoculated onto a surface, and then the bacteria are collected from the surface and examined as to whether they can form colonies on the agar plate [1]. In the present study, the presence of bacteria was examined by direct detection. Our study is based on FTIR PAS with an interferometer cantilever detector [2]. Our work discusses the possibility of identifying and distinguishing the presence of different bacteria (Staphylococcus epidermidis and Pseudomonas aeruginosa) and the possibility to evaluate the crystallization processes on the pressed calcium phosphate surface.

Keywords
FTIR-PAS, FTIR-DRIFT, Pseudomonas aeruginosa, Staphylococcus epidermidis, in vitro, Principal Component Analysis (PCA), Nanocrystalline apatite (NA).
DOI
10.4028/www.scientific.net/KEM.758.273
Hyperlink
https://www.scientific.net/KEM.758.273

Brangule, A., Skadiņš, I., Reinis, A., Gross, K., Kroiča, J. In Vitro Characterization Perspectives Using Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR PAS) and Diffuse Reflectance Infrared Spectroscopy (DRIFT). In: Key Engineering Materials, France, Toulouse, 25-27 October, 2017. Switzerland: Unknown, 2017, pp.273-277. ISSN 1013-9826. e-ISSN 1662-9795. Available from: doi:10.4028/www.scientific.net/KEM.758.273

Publication language
English (en)