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Publikācija: Study of Potential PCR Inhibitors in Drinking Water for Escherichia Coli Identification

Publication Type Scientific article indexed in SCOPUS or WOS database
Funding for basic activity Other research projects
Defending: ,
Publication language English (en)
Title in original language Study of Potential PCR Inhibitors in Drinking Water for Escherichia Coli Identification
Field of research 1. Natural sciences
Sub-field of research 1.6 Biological sciences
Authors Brigita Daļecka
Linda Mežule
Keywords Polymerase chain reaction, inhibitors, chlorine, humic acids, iron, Escherichia coli
Abstract In the last few decades, the polymerase chain reaction (PCR) has become one of the most powerful molecular biological tools. However, the PCR is an enzymatic reaction and therefore sensitive to inhibitors which may occur in drinking water samples. In this work, the possible inhibition effect of chlorine, humic acids, and iron for real-time PCR (qPCR) efficiency was studied and the environmental sample from drinking water treatment system before iron removal was selected and analysed. The results demonstrated that the highest concentrations of humic acids (5 and 1 mg l-1) and iron (4 mg l-1) inhibited the PCR reaction while no effect of chlorine was observed. The analysis of the environmental sample with spiked Escherichia coli cells demonstrated reduction efficiency of the average threshold cycle (Ct) values compared with control dilution series determining the possible inhibition for qPCR assay.
DOI: 10.15159/AR.18.118
Hyperlink: https://dspace.emu.ee/xmlui/handle/10492/3944 
Reference Daļecka, B., Mežule, L. Study of Potential PCR Inhibitors in Drinking Water for Escherichia Coli Identification. Agronomy Research, 2018, Vol.16, Special Iss.2, pp.1351-1359. ISSN 1406-894X. Available from: doi:10.15159/AR.18.118
Additional information Citation count:
  • Scopus  0
ID 27971