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Publikācija: In Vivo and in Vitro Study of a Novel Nanohydroxyapatite Sonocoated Scaffolds for Enhanced Bone Regeneration

Publication Type Scientific article indexed in SCOPUS or WOS database
Funding for basic activity Research project
Defending: ,
Publication language English (en)
Title in original language In Vivo and in Vitro Study of a Novel Nanohydroxyapatite Sonocoated Scaffolds for Enhanced Bone Regeneration
Field of research 2. Engineering and technology
Sub-field of research 2.5 Materials engineering
Research platform Materials, Processes, and Technologies
Authors Julia Rogowska-Tylman
Jānis Ločs
Ilze Salma
Bartosz Wozniak
Māra Pilmane
Vita Zālīte
Jacek Wojnarowicz
Aleksandra Kędzierska-Sar
Tadeusz Chudoba
Karol Szlązak
Adrian Chlanda
wojciech Swieszkowski
Aharon Gedanken
Witold Lojkowski
Abstract There still remains a need for new methods of healing large bone defects, i.e., gaps in bone tissue that are too big to naturally heal. Bone regrowth scaffolds can fill the bone gap and enhance the bone regeneration by providing cells with a support to for new tissue formation. Coating of the scaffolds surface with nanocrystalline hydroxyapatite may enhance the osteoinductivity or osteoconductivity of suchs caffolds. Here we present the sonocoating method to coat scaffolds with bioactive hydroxyapatite nanoparticles. We show a method, where the material to be coated is immersed in a colloidal suspension of nanoparticles with mean sizes of 10 nm and 43 nm in water, and high power ultrasound waves are applied to the suspension for 15 min at 30°C. High power ultrasounds lead to growth of cavitation bubbles in liquid, which implode at acritical size. The implosion energy propels the nanoparticles towards the material surface, causing their attachment to the scaffold. Using this technique, we produced a uniform layer of nanohydroxyapatite particles of thickness in the range 200 to 300 nm on two types of scaffolds: a porous β-TCP ceramic scaffold and a 3D-printed scaffold made of PCL fibers. In vivo tests in rabbits confirmed that the novel coating strongly stimulated new bone tissue formation, with new bone tissue occupying 33% for the nHAP-coated PCL scaffold and 68% for the nHAP-coated β-TCP after a 3-month test. The sonocoating method leads to formation of a bioactive layer on the scaffolds at temperature close to room temperature, very short time and in water. It is a green technological process, promising for bone tissue regeneration applications.
DOI: 10.1016/j.msec.2019.01.084
Hyperlink: https://www.sciencedirect.com/science/article/pii/S0928493118314243?via%3Dihub 
Reference Rogowska-Tylman, J., Ločs, J., Salma, I., Wozniak, B., Pilmane, M., Zālīte, V., Wojnarowicz, J., Kędzierska-Sar, A., Chudoba, T., Szlązak, K., Chlanda, A., Swieszkowski, w., Gedanken, A., Lojkowski, W. In Vivo and in Vitro Study of a Novel Nanohydroxyapatite Sonocoated Scaffolds for Enhanced Bone Regeneration. Materials Science & Engineering: C, 2019, Vol. 99, pp.669-684. ISSN 0928-4931. Available from: doi:10.1016/j.msec.2019.01.084
Additional information Citation count:
ID 29976