The amber nano fibres are bio-active composites which could be applied in three-dimensional printing. The idea of using the amber for creating a new 3D printing material, including technological composition of the innovative amber nano and micro fibres is based on the hypothesis that organic compounds of succinite have an effective impact on living cells, including reparative, stimulating, for sedative effects as well protective properties from electro-magnetic field. To produce amber nano fibres, bioactive components were used that were isolated from the succinite and tested (in vitro) in the scientific laboratories. Three-dimensional printing is an emerging technology, which has been initially used to design and generate three-dimensional structures mainly for transplantation therapies. This process, which can be used for a variety of polymers, is becoming an increasingly essential component of biotechnological advancements. This article discusses the foundations of this technology, namely the processes used in conventional three-dimensional printing; it also discusses tissue engineering, a discipline of which new implementations of this technology are used as bio printing. The shortcomings of tissue engineering are addressed, including the failure of existing technology to manufacture nanofiber-based constructs used in blood vessels, cartilage, artery valves, tendon, cardiac muscle valves, muscle, and cornea. From this need for nanofiber processing, electrospinning is proposed as a possible roadmap for imminent tissue engineering three-dimensional printing technologies, and finally, the latest integration of this technology with three-dimensional printing is addressed, highlighting the existing shortcomings in maintaining mandatory nano resolutions.