Influence of UV Radiation on Immobilization of Yeast Cells on the Surfaces of Mg-and Ti-Originated Alloys and Their CaP Coatings

19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC 2023). IFMBE Proceedings. Vol.89 2023
Marks Gorohovs, Anna Bystrova, Jurijs Dehtjars, Marina Romanova, Gaļina Hrustaļova, Jurgen Schmidt, Alina Vladescu

For the past few decades, many researches have been devoted to study and develop new biocompatible materials for medical implants. MgCa1 and TiAl6 V4 are examples of such materials. It is possible to adjust their physical and chemical parameters by coating the surface with calcium phosphates (CaP). In turn, exposing materials to UV radiation influencing the surface charge may contribute to cell immobilization and thus materials biocompatibility. The purpose of this study was to investigate how UV radiation influences the immobilization of yeast cells on the surfaces of Mg-and Ti-originated alloys and their CaP coatings. MgCa1 and TiAl6 V4 samples with different coatings (uncoated, CaP, Ca+ and variations doped with Zn or Ga) were used. Surface roughness, FTIR spectras and surface electric potential (via electron work function measurements) were assessed before and after UV irradiation (for 30 and 60 min). S. cerevisiae were immobilized on the surfaces of samples and assessed its coverage using fluorescent microscopy. Adherence of yeast cells was found to be more prominent for Ti samples, than to Mg samples. There was no conclusive link discovered between the samples’ surface roughness and yeast cells’ adherence to it. UV irradiation does not affect the chemical structure of the samples. Overly, UV irradiation has improved the cell immobilization on Mg-and Ti-originated alloys with different CaP coating, exceptions are only coatings with Zn or Ga dopings. Non-coated Mg-and Ti-originated alloys showed the best adherence of yeast cells.

Keywords
Coatings | Magnesium alloy | surface charge | Titanium alloy | UV radiation | yeast cells
DOI
10.1007/978-3-031-37132-5_33
Hyperlink
https://link.springer.com/chapter/10.1007/978-3-031-37132-5_33

Gorohovs, M., Bystrova, A., Dehtjars, J., Romanova, M., Hrustaļova, G., Schmidt, J., Vladescu, A. Influence of UV Radiation on Immobilization of Yeast Cells on the Surfaces of Mg-and Ti-Originated Alloys and Their CaP Coatings. In: 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC 2023). IFMBE Proceedings. Vol.89, Latvia, Liepaja, 12-14 June, 2023. Cham: Springer, 2023, pp.261-268. ISBN 9783031371318. e-ISBN 978-3-031-37132-5. ISSN 1680-0737. Available from: doi:10.1007/978-3-031-37132-5_33

Publication language
English (en)