Laser Speckle Imaging for Visualization of Hidden Effects for Early Detection of Antibacterial Susceptibility in Disc Diffusion Tests
Frontiers in Microbiology
2023
Ilya Balmages,
Aigars Reinis,
Svjatoslavs Kistkins,
Dmitrijs Bļizņuks,
Emilija Vija Plorina,
Alexey Lihachev,
Ilze Lihacova
Rapid identification of effective antibiotic treatment is crucial for increasing patient survival and preventing the formation of new antibiotic-resistant bacteria due to preventative antibiotic use. Currently utilized “gold standard” methods require 16–24 h to determine the most appropriate antibiotic for the patient’s treatment. The proposed technique of laser speckle imaging with subpixel correlation analysis allows for identifying dynamics and changes in the zone of inhibition, which are impossible to observe with classical methods. Furthermore, it obtains the resulting zone of inhibition diameter earlier than the disk diffusion method which is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). These results could improve mathematical models of changes in the diameter of the zone of inhibition around the disc containing the antimicrobial agent, thereby speeding up and facilitating epidemiological analysis. Copyright © 2023 Balmages, Reinis, Kistkins, Bliznuks, Plorina, Lihachev and Lihacova.
Keywords
antibiotic resistance; antibiotic sensitivity; Article; bacterium detection; correlation analysis; disk diffusion; Enterobacter aerogenes; Escherichia coli; gold standard; image processing; mathematical model; mathematical phenomena; nonhuman; procedures, parameters and devices; spatiotemporal analysis; visualization; zone of inhibition
DOI
10.3389/fmicb.2023.1221134
Hyperlink
https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1221134/full
Balmages, I., Reinis, A., Kistkins, S., Bļizņuks, D., Plorina, E., Lihachev, A., Lihacova, I. Laser Speckle Imaging for Visualization of Hidden Effects for Early Detection of Antibacterial Susceptibility in Disc Diffusion Tests. Frontiers in Microbiology, 2023, Vol. 14, Article number 1221134. ISSN 1664-302X. Available from: doi:10.3389/fmicb.2023.1221134
Publication language
English (en)