Bio-oils of different hardwood species were obtained in a 2-stage ablative pyrolysis reactor. The GC/MS analysis of the bio-oils has shown a different content of products of degradation of carbohydrates and lignin depending on the wood species. Bio-oils were dispersed in water and separated to soluble (Py-C) and insoluble (Py-L) fractions. The fractions were characterised by the Py-GC/MS method. It is shown that the Py-C fraction contains, besides products of carbohydrate origin, phenolic products, which are represented mainly by syringyl derivatives. The antioxidant properties of Py-L and Py-C isolated from bio-oil of different wood species were characterised using three assays such as ABTS●+/ DPPH●/superoxide radical scavenging.