It is well known that both human body, as well as vegetable oils is subjected to aging processes in the presence of free radicals. Variable antioxidants are used to reduce the impact of the; in order to study new antioxidants a wide range of method are used. This paper reviews literature data on determinating antioxidant activity both for lipophilic and hydrophilic antioxidants. Common used ABTS or DPPH tests as well as alternative methods are described. The literature rewiev is focused on the new tendences of performence for classical methods, mainly, involving electrchemistry instead of chemical oxidation or organization of analysis in flow system. The use of potenciometry, amperometry and spin resonance spectroscopy for determination of antioxidant activity is described, too. All the methods are clasified according to the phases were active compounds (DPPH, ABTS etc.) or radical generators are located. The most common are tests were all the compounds (including sample) are in one phase – liquid. Nevertheless a few methods are described were active compound is located in solid phase or in gase phase.