Bioactive materials implanted into bone defects bonds to living bone without encapsulation by a fibrous tissue. Bioactive materials form bone-like apatite layer on their surfaces and bonds to living bone through it. The structure and composition of formed apatite is similar to apatite in living bone. The bioactivity of material, which is affected by surface properties and morphology, can be evaluated in vitro in simulated body fluid (SBF). For the biomimetic apatite formation on materials, chemical species for induction of the nucleation should be included at the surfaces. Titanium dioxide (TiO2) ceramics can be used as one of bioactive implant materials. In the present work, TiO2 samples were obtained from nanopowder by uniaxial pressing and thermally treating at different temperature regimes. UV-light irradiated and non-iradiated samples were soaked in SBF for various periods of time. The surface morphology and composition of samples before and after soaking in SBF were studied with SEM, XRD, EDX, FTIR and Raman spectroscopy. Thermal treatment at higher temperatures reduces porosity, BET specific surface area and increases amount of rutile amount in the samples, which decreases apatite-forming ability on TiO2 surface.