Small and medium-sized enterprises have an important role for economic development and creation of new employment opportunities, therefore, one of the most essential issues is to support these types of enterprises. One of the financial instruments, that is co-financed by the European Union (EU) structural funds, relates to business incubators. Business incubators are one of the solution opportunities that have already been implemented in different countries, in order to develop new enterprises with strong innovative components. Business incubators foster development of new companies at the early-stage and help them to survive and develop during the start-up period, when high risks exist. The aim of the research is to analyze the importance in setting up new enterprises, special attention paying to the use of the structural funds of the European Union. This paper analyzes the following issues: core of business incubator, availability of the EU structural funds, a framework for results based on the received support. The research includes EU financed business incubator work results, which can illustrate a new company setting up process and its promotion prospects.