Calcium deficient strontium and fluorine co-substituted hydroxyapatite (SrFCDHAp, Ca10- xSrx(PO4)6-y(HPO4)y(OH)2-zFz) was synthesized by 2 different wet chemical precipitation methods – pH cycling and nitrate salt method. Dried and non-calcined SrFCDHAp powders were analysed by X-ray powder diffractometry (XRD) and Fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Calcined powders were analysed by XRD and FT-IR. Using XRD it was shown that the thermally treated samples contain not only the apatite phase, but also TCP phase, which indirectly implies that the synthesized products are Ca-deficient. Maximums at 750 cm-1 and 3485 cm-1 in FT-IR spectrum approved fluoride incorporation into SrFCDHAp structure, while maximum at 880 cm-1 (in non-calcined powders spectrum) confirms that the calcium deficient products were obtained. pH cycling method was the most optimal method to incorporate strontium ions into SrFCDHAp structure (10.9±0.2 mass%), while nitrate salt method allowed to incorporate more fluorine atoms (2.5±0.2 mass%). The size of the crystallites of the powdered samples were larger for products obtained with pH cycling (29 ± 3 nm) than using the nitrate salt synthesis method (19±3 nm).