In order to find fast and simple techniques for biodiesel fuel quality monitoring, total luminescence and conventional spectra in region 400-800 nm of biodiesel before heating and during the heating at 200 °C and 250 °C were investigated. The fluorescence characteristics during biodiesel degradation changed dramatically and the total luminescence spectra are very sensitive visual indicator for quality. In the long wave region not only degradation of pigments, but also formation of pigment-like substances were observed. For practical quantitative monitoring the determination of initial rate of reactions in growing regions of fluorescence should be valuable.