In the last 10 years recycling of various materials and metal recovery from waste with low cost biosorbents or agricultural biomass has become popular trend. Lignocellulosic biomass is regarded as a sustainable resource for biofuel production. In this process, lignocellulosic biomass is partly degraded during chemical or biological hydrolysis, as a result, these agricultural waste materials usually present a disposal problem and have no economic value. Therefore, reuse of lignocellulosic waste materials as inexpensive and alternative sorbent for heavy metals in polluted aqueous solution. The aim of this research was to evaluate the applicability of hydrolysed biomass waste for zinc removal from water solution. To evaluate the potential use of lignocellulosic biomass for biosorption, experiments were performed with dry, washed from organic matter and hydrolysed hay. The results showed that hydrolysed hay have lower biosorption capacity than washed and dried hay, however, it still can be used as a low-cost biosorbent for the removal of Zn in polluted aqueous solutions, because it showed relatively high cink sorption capasity (336-391 mg g-1). © 2017, Eesti Pollumajandusulikool. All rights reserved.