The polybutylene succinate biopolymer 3D printing application has been assessed for the mean of the environmental impact and inventory analysis compared to the traditionally widely used polylactide (PLA) polymer. The waste, pollution, and energy impact were assessed for those materials. The proposed polybutylene succinate polymer filament was processed from the commercial pellets by melt blending extrusion and Fused Filament Fabrication (FFF) tests. The samples were printed using the heuristic optimization procedure for printing fabrication. The specimens were prepared by modifying printing conditions – velocity, temperature, retraction, and other processing parameters. Several shapes specimens have been tested – bars, double dog bones, and other structures. The specimens have been assessed for mechanical response in tension conditions by applying external impact factors and humidity (25%, 100%).