In recent years, it has become topical to study the concept of physical literacy in early childhood, based on empirical evidence of how the formation of skills takes place at this stage. The question remains open for the researchers -how to standardize and simply assess the physical literacy given its broad framework. One of the definitions of physical literacy explains the essence and importance of this concept in a person's life as internal motivation, confidence, competence in physical activities, knowledge and the person's own responsibility for applying it in practice. The systematic literature review was conducted with the aim of identifying a safe, sustainable and easy-to-apply physical literacy assessment tool for preschool children aged 3 to 7 years. Methodology.The systematic literature review protocol includes sources from such databases as EBSCO host (MEDLINE, Eric), Google Scholar, Science Direct, ERIH PLUS, Scopus and Web of Science for the period 2018 –2024, which title, summary and keywords meets the certain criteria and content includes information on physical literacy assessment tools for the age specified. The content analysis was performed for the identified tools to find out their relevance for the assessment of physical, affective, cognitive, and participatory domains of physical literacy. Results. As a result of the content analysis, 26 different tools for assessing physical literacy in pre-school age were identified. The most commonly used instrument is the Test of Gross Motor Development by Ulrich. For the assessment of all four domains of physical literacy at preschool age, three tools were identified –Pre -PLAy, a set of tools based on the guidelines of Canadian Assessment of Physical Literacy, and the Nine-Step Assessment Approach based on Australian Physical Literacy Framework. Conclusions.The content analysis led to the conclusion that there is a small number of unified universal tools that can be appliedat preschool age and would include four domains of physical literacy. The research directions of physical literacy assessment tools at pre-school age are the following: development and validation of a universal tool or tool sets, application of smart technologies, virtual reality and remote assessment possibilities in the evaluation process.