Sorptive Immobilization and Subsequent Release of Hen Egg Lysozyme
2013
Valentīna Krilova, Renāte Steika

For lysozyme immobilization, synthesized carboxylic ion-exchanger has been used. Lysozyme immobilization was performed both from model solution and from diluted hen egg white. Enzymatic activity of lysozyme containing solutions was evaluated by Micrococcus lysodeiktikus cell lysis. Reversibly conjugated lysozyme release was ascertained to depend on the contacted medium composition. Lysozyme release from conjugates was stable for the duration of experiment (11 days). To evaluate the stability of conjugate over time, samples were stored at ambient temperature for 72 days. Lytic activity of desorbates was practically unchanged as compared to the initial activity.


Atslēgas vārdi
lysozyme, immobilization, sorption, lytic activity.

Krilova, V., Steika, R. Sorptive Immobilization and Subsequent Release of Hen Egg Lysozyme. Materiālzinātne un lietišķā ķīmija . Nr.27, 2013, 27.-31.lpp. ISSN 14077353.

Publikācijas valoda
English (en)
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