Business ecosystem is a relatively new concept in business field of research, which is necessary for clarification. The purpose of this research is to find out theoretical concept of business ecosystem and them containing elements, as well as the healthy business ecosystem and it assessment in bank. In order to achieve the results of the present research, the qualitative overview of the scientific literature on business ecosystem has been performed, as well as the concept of business ecosystem. Business ecosystem concept was used for the assessment of qualitative research SOFTWARE as an NVivo. Based on the NVivo results obtained and the information collected of business ecosystem concept was shown using a tool TagCloud. According to the findings and the definition of ISO standards development, the authors stated the definition of business ecosystem. With the help of NVivo softvare and TagCloud the authors found frequently most used words that describe the business ecosystem concept as well as to create the new definition of business ecosystem.