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Publikācija: Hydrogen-Producing Escherichia Coli Strains

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Publikācijas valoda English (en)
Nosaukums oriģinālvalodā Hydrogen-Producing Escherichia Coli Strains, Overexpressing Lactose Permease: FT-IR Analysis of the Lactose-Induced Stress
Pētniecības nozare 2. Inženierzinātnes un tehnoloģijas
Pētniecības apakšnozare 2.1. Būvniecības un transporta inženierzinātnes
Autori Māra Grūbe
Ilze Dimanta
Marita Gavare
Inese Strazdiņa
J. Liepiņš
Tālis Juhna
Uldis Kalnenieks
Atslēgas vārdi Biohydrogen fermentation; Escherichia coli; FT-IR spectroscopy; glycogen; lactose; lactose permease
Anotācija Lactose permease gene (lacY) was overexpressed in the septuple knock-out mutant of Escherichia coli, previously engineered for hydrogen production from glucose. It was expected that raising the lactose transporter activity would elevate the intracellular lactose concentration, inactivate the lactose repressor, induce the lactose operon, and in result, stimulate the overall lactose consumption and conversion. However, overexpression of the lactose transporter caused a considerable growth delay of the recombinant strain on lactose, resembling to some extent the ‘lactose killing’ phenomenon, described by Dykhuizen and Hartl [1]. Therefore, the recombinant strain was subjected to selection on lactose-containing media. Selection on plates with 3% lactose yielded a strain with a decreased content of the recombinant plasmid, yet with an improved ability to grow and produce hydrogen on lactose. Macromolecular analysis of its biomass by means of FT-IR spectroscopy demonstrated that increase of the cellular polysaccharide content might contribute to the adaptation of E. coli to lactose stress.
DOI: 10.1002/bab.1128
Atsauce Grūbe, M., Dimanta, I., Gavare, M., Strazdiņa, I., Liepiņš, J., Juhna, T., Kalnenieks, U. Hydrogen-Producing Escherichia Coli Strains, Overexpressing Lactose Permease: FT-IR Analysis of the Lactose-Induced Stress. Biotechnology and Applied Biochemistry, 2013, Vol.61, Iss.2, 111.-117.lpp. ISSN 1470-8744. Pieejams: doi:10.1002/bab.1128
Papildinformācija Citējamību skaits:
ID 17786