Baltic amber - succinite due to its unique chemical composition and content of various biologically active compounds, especially succinic acid, can be successfully applied in the development of new biomaterials. In our previous studies, the processing method of succinite has been developed, which allow to obtain pure succinite powder without impurities and particles size >5µm (176µm, 95%). In vivo tests (Albino guinea pig), showed that water suspension of succinite powder doesn’t cause skin irritation and organism sensitization, but increases growth of collagen (types I and III) and elastin in the skin, in blood vessel’s walls and muscle tissue. For further research grinding method should be optimized in order to reduce particle size up to 3µm, to obtain spherical shape of particles and save initial chemical composition. It was achieved by a two-stage wet grinding in water media. The first (preground) with 3 or 5 mm YTZ® (Yttrium Stabilized Zirconia) during 240min., then changing grinding media to 0.4-0.6mm YTZ®, 30min. Particle size of the obtained succinite powder is 3µm (95%) with spherical shape and unchanged chemical composition, which was confirmed by atomic force microscopy and Fourier transform infrared spectroscopy.