Zinātniskās darbības atbalsta sistēma
Latviešu English

Publikācija: Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation

Publikācijas veids Zinātniskais raksts, kas indeksēts Web of science un/vai Scopus datu bāzē
Pamatdarbībai piesaistītais finansējums Nav zināms
Aizstāvēšana: ,
Publikācijas valoda English (en)
Nosaukums oriģinālvalodā Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation
Pētniecības nozare 1. Dabaszinātnes
Pētniecības apakšnozare 1.4. Ķīmija
Autori N. Kronqvist
M. Otikovs
V. Chmyrov
G. Chen
M. Andersson
K. Nordling
M. Landreh
M. Sarr
H. Jörnvall
S. Wennmalm
J. Widengren
Q. Meng
A. Rising
D. Otzen
S.D. Knight
Kristaps Jaudzems
J. Johansson
Atslēgas vārdi
Anotācija The mechanisms controlling the conversion of spider silk proteins into insoluble fibres, which happens in a fraction of a second and in a defined region of the silk glands, are still unresolved. The N-terminal domain changes conformation and forms a homodimer when pH is lowered from 7 to 6; however, the molecular details still remain to be determined. Here we investigate site-directed mutants of the N-terminal domain from Euprosthenops australis major ampullate spidroin 1 and find that the charged residues D40, R60 and K65 mediate intersubunit electrostatic interactions. Protonation of E79 and E119 is required for structural conversions of the subunits into a dimer conformation, and subsequent protonation of E84 around pH 5.7 leads to the formation of a fully stable dimer. These residues are highly conserved, indicating that the now proposed three-step mechanism prevents premature aggregation of spidroins and enables fast formation of spider silk fibres in general.
DOI: 10.1038/ncomms4254
Hipersaite: http://www.nature.com/ncomms/2014/140210/ncomms4254/full/ncomms4254.html 
Atsauce Kronqvist, N., Otikovs, M., Chmyrov, V., Chen, G., Andersson, M., Nordling, K., Landreh, M., Sarr, M., Jörnvall, H., Wennmalm, S., Widengren, J., Meng, Q., Rising, A., Otzen, D., Knight, S., Jaudzems, K., Johansson, J. Sequential Ph-Driven Dimerization and Stabilization of the N-Terminal Domain Enables Rapid Spider Silk Formation. Nature Communications, 2014, No.5, 3254.-3254.lpp. e-ISSN 2041-1723. Pieejams: doi:10.1038/ncomms4254
Papildinformācija Citējamību skaits:
ID 19204