This experimental study assesses the influence of ignition timing on emissions from a production four cylinder port injection spark ignition engine. The aim of this research was to evaluate the necessity of ignition timing correction when the regular gasoline vehicle is being adapted for the use of E85 fuel. Tests were conducted in the Alternative Fuels Research Laboratory of Latvia University of Agriculture in December 2013. The engine was fuelled with the ethanol-gasoline blend E85 or the commercial gasoline A95. The engine was tested within a vehicle in a chassis dynamometer in steady state conditions, which resemble driving at 50 kmh-1 and 90 kmh-1. The original engine control unit was replaced with a programmable one. Engine-out and tailpipe exhaust gas samples were taken and analysed with a FTIR-type analyser AVL SESAM. Carbon monoxide (CO), unburned hydrocarbons (HC), nitrogen oxides (NOx), acetaldehyde and unburned ethanol emission volumetric share is presented. CO, HC and acetaldehyde emissions were not affected by variation of the ignition timing within the tested range. NOx and ethanol emissions were reduced with the ignition timing retard. The emissions of CO, HC and NOx were reduced, when the engine was fuelled with the E85 fuel, comparing with the gasoline use. Ignition timing, optimized for the gasoline, was found suitable for the E85 fuel from the emission analyses point.