Crystallized Nano-Sized Alpha-Tricalcium Phosphate from Amorphous Calcium Phosphate: Microstructure, Cementation and Cell Response
Biomedical Materials 2015
Linda Vecbiškena, Kārlis-Agris Gross, Una Riekstiņa, Thomas Chung-Kuang Yang

New insight on the conversion of amorphous calcium phosphate (ACP) to nano-sized alpha tricalcium phosphate (α-TCP) provides a faster pathway to calcium phosphate bone cements. In this work, synthesized ACP powders were treated with either water or ethanol, dried, crystallized between 700 and 800 °C, and then cooled at different cooling rates. Particle size was measured in a scanning electron microscope, but crystallite size calculated by Rietveld analysis. Phase composition and bonding in the crystallized powder was assessed by x-ray diffraction and Fourier-transform infrared spectroscopy. Results showed that 50 nm sized α-TCP formed after crystallization of lyophilized powders. Water treated ACP retained an unstable state that may allow ordering to nanoapatite, and further transition to β-TCP after crystallization and subsequent decomposition. Powders treated with ethanol, favoured the formation of pure α-TCP. Faster cooling limited the growth of β-TCP. Both the initial contact with water and the cooling rate after crystallization dictated β-TCP formation. Nano-sized α-TCP reacted faster with water to an apatite bone cement than conventionally prepared α-TCP. Water treated and freeze-dried powders showed faster apatite cement formation compared to ethanol treated powders. Good biocompatibility was found in pure α-TCP nanoparticles made from ethanol treatment and with a larger crystallite size. This is the first report of pure α-TCP nanoparticles with a reactivity that has not required additional milling to cause cementation.


Atslēgas vārdi
amorphous calcium phosphate, alpha tricalcium phosphate, Rietveld refinement, cell response, bone cements, regenerative medicine
DOI
10.1088/1748-6041/10/2/025009
Hipersaite
http://iopscience.iop.org/1748-605X/10/2/025009/

Vecbiškena, L., Gross, K., Riekstiņa, U., Chung-Kuang Yang, T. Crystallized Nano-Sized Alpha-Tricalcium Phosphate from Amorphous Calcium Phosphate: Microstructure, Cementation and Cell Response. Biomedical Materials, 2015, Vol.10, No.2, 1.-10.lpp. ISSN 1748-6041. e-ISSN 1748-605X. Pieejams: doi:10.1088/1748-6041/10/2/025009

Publikācijas valoda
English (en)
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