The paper studied the chemical and microbiological nature of the produced water. The aim of study is to describe the interdependence of the chemical composition and microbiological activity of the produced water in the combination with various types and amounts of shungite for the treatment of produced water. For the first time produced water from Kumkol field was studied. The practical value of research is to develop a methodology where the implementation of biotechnology for a high degree of purification is given. From a practical point of view carbonate-shale shungit is of interest because it provided the largest decline in chlorides (1.7 %) and the smallest increase in sulphates (13.5 %). Use of all shungite has a beneficial health effect on the process of water purification, as coliform bacteria were not found in experimental samples. Based on the results from microbiological analysis the most beneficial effect on the activity of the studied taxa affects carbonate-shale shungite, and relatively neutral stance on this characteristic has Russian shungite.