The new fuel ‘Pro Diesel’ that contains hydrotreated vegetable oil (HVO) was recently introduced in Baltic market. It raised some interest on performance of the new fuel among fleet and individual consumers. The authors evaluated and compared performance of modern M1 class automobile, using regular fossil diesel fuel and Pro Diesel fuel. Torque, power and fuel consumption of the vehicle have been evaluated on chassis dynamometer, in steady state and driving cycle mode. Depending on test conditions, engine power and torque was increased up to 2%, and fuel consumption reduced up to 3.9%, when diesel fuel/ HVO blend was used.