A typical phenomenon in concrete structures is crack formation. Shrinkage cracks may appear in early stage of concrete. In late age cracks may appear due to process of serviceability and external weather conditions. Larger cracks interfere structural integrity, microcracks may result in durability problems as increase of water absorption, which results in concrete degradation and reinforcement corrosion. Manual maintenance of the concrete structure is costly and labour consuming; therefore, functionality of self-healing phenomenon of concrete structures has been inspected. In this study self-healing phenomenon of bacteria-based concrete has been investigated. Calcium lactate and yeast medium with spore-forming alkaliphilic bacteria Bacillus Lentus have been chosen. Bacillus Lentus spores with medium were integrated (by using vacuum technique) into expanded clay granules (LECA) with a size of 2-4 mm. Direct addition of bacteria (without immobilization) in concrete limits the survival of bacteria from 1 to 2 months. In this study, to increase viability of bacteria at high pH media in concrete and thus the time-related functionality of self-healing agents, bacteria were immobilized in LECA.