The study presents an experimental investigation of the impact response of hybrid biocomposites that are based on polypropylene homopolymer HP400R reinforced with man-made cellulose fibres and cellulose microfiller. Man-made cellulose fibres, such as rayon tire cord yarn (Cordenka) and soft wood microfibres (Weho 500) were added to the polypropylene matrix with different weight contents. Composite specimens were manufactured by injection moulding. Absorbed energies and maximum impact forces of biocomposite were determined for each filling ratio of Cordenka and Weho 500 and have been selected as the parameters of the study. It was performed by the method of planning of the experiment and response surface method. Results showed that combination of two types of fillers improved impact properties. Response surface plots reveal that energy and maximum load of biocomposites increases with increasing content of reinforcements, however, the man-made cellulose Cordenka fibres have dominant influence on the obtained results. An addition of the Cordenka fibres increased the absorbed energy by ~372 % and ~243 % in comparison with the unreinforced polypropylene matrix and composite reinforced with 30 wt. % of Weho fibres. The substitution of Cordenka fibres with soft wood fibres gave increase only by ~72 % and ~27 % in comparison with the unreinforced polypropylene matrix and composite reinforced with 30 wt. % of Cordenka fibres.