Magnetic Levitational Bioassembly of 3D Tissue Construct in Space
Science Advances
2020
Vladislav Parfenov,
Yusef Khesuani,
Stanislav Petrov,
Pavel Karalkin,
Elizaveta Koudan,
Elizaveta Nezhurina,
Frederico Pereira,
Alisa Krokhmal,
Anna Gryadunova,
Elana Bulanova,
Igor Vakhrushev,
Igor Babichenko,
Vladimirs Kasjanovs,
Oleg Petrov,
Mikhail Vasiliev,
Kenn Brakke,
Sergei Belousov,
Timofei Grigoriev,
Egor Osidak,
Ekaterina Rossiyskaya,
Ludmila Buravkova,
Oleg Kononenko,
Utkan Demirci,
Vladimir Mironov
Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine.
Atslēgas vārdi
simulated microgravity; growing tissues; cartilage; design; growth; cells
DOI
10.1126/sciadv.aba4174
Hipersaite
https://advances.sciencemag.org/content/6/29/eaba4174
Parfenov, V., Khesuani, Y., Petrov, S., Karalkin, P., Koudan, E., Nezhurina, E., Pereira, F., Krokhmal, A., Gryadunova, A., Bulanova, E., Vakhrushev, I., Babichenko, I., Kasjanovs, V., Petrov, O., Vasiliev, M., Brakke, K., Belousov, S., Grigoriev, T., Osidak, E., Rossiyskaya, E., Buravkova, L., Kononenko, O., Demirci, U., Mironov, V. Magnetic Levitational Bioassembly of 3D Tissue Construct in Space. Science Advances, 2020, Vol. 6, No. 29, eaba4174. e-ISSN 2375-2548. Pieejams: doi:10.1126/sciadv.aba4174
Publikācijas valoda
English (en)