During the COVID-19 pandemic, the provision of appropriate protective clothing for medical staff who must continue to perform their duties at this period is of particular importance. Physiotherapists are not allowed to suspend working during this time either, and the specifics of their work is close contact with the patient within various movements during the therapy process. Inadequate (intended for general use) protective clothing affects work capacity in the aspects of movement restriction, thermal comfort and thereby increasing energy consumption. For the design and supply of personal protective equipment (PPE) and protective clothing of the best possible quality, PPE should be explored in a systemic approach (holistics) to assess anthropometric fit and ergonomics, material properties and thermal performance. This study investigated anthropometric characteristics of target group representatives; sizing, fit and design flaws of protective suits for physiotherapists; provision of mobility in the protective clothing of physiotherapists; the protective suit fabric layer interaction with the human body and the importance of subjective evaluation of fit and ergonomics of PPE (made by specialists and wearers). The results of the study promote the usage of objective knowledge of anthropometry and ergonomics as well as subjective assessment findings in the development of protective suits for physiotherapists.