Crypthecodinium Cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
Marine Drugs 2022
Elīna Didrihsone, Konstantīns Dubencovs, Māra Grūbe, Kārlis Švirksts, Anastasija Šuleiko, Artūrs Šuleiko, Juris Vanags

Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried out with the use of yeast extract (YE) as a nitrogen source. In the present study, alternative carbon and nitrogen sources were studied: the extraction ethanol (EE), remaining after lipid extraction, as a carbon source, and dinoflagellate extract (DE) from recycled algae biomass C. cohnii as a source of carbon, nitrogen, and vitamins. In mediums with glucose and DE, the highest specific biomass growth rate reached a maximum of 1.012 h−1, while the biomass yield from substrate reached 0.601 g·g−1 . EE as the carbon source, in comparison to pure ethanol, showed good results in terms of stimulating the biomass growth rate (an 18.5% increase in specific biomass growth rate was observed). DE supplement to the EE-based mediums promoted both the biomass growth (the specific growth rate reached 0.701 h−1 ) and yield from the substrate (0.234 g·g−1 ). The FTIR spectroscopy data showed that mediums supplemented with EE or DE promoted the accumulation of PUFAs/docosahexaenoic acid (DHA), when compared to mediums containing glucose and commercial YE.


Atslēgas vārdi
Biomass recycling; Crypthecodinium cohnii; Dinoflagellate extract; FTIR spectroscopy; Omega-3 fatty acid
DOI
10.3390/md20010068
Hipersaite
https://www.mdpi.com/1660-3397/20/1/68

Didrihsone, E., Dubencovs, K., Grūbe, M., Švirksts, K., Šuleiko, A., Šuleiko, A., Vanags, J. Crypthecodinium Cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass. Marine Drugs, 2022, Vol. 20, No. 1, Article number 68. ISSN 1660-3397. Pieejams: doi:10.3390/md20010068

Publikācijas valoda
English (en)
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