Zinc-Substituted Hydroxyapatite-Bacteriophage Complexes for Complementary Antibacterial Properties
Colloids and Surfaces A: Physicochemical and Engineering Aspects 2025
Dace Rezevska, Līga Stīpniece, Lelde Rubene, Marika Ščeglova, Sandis Mauriņš, Renāts Vasiļjevs, Kārlis Rācenis, Juta Kroiča

The increasing resistance of bacteria to antibiotics poses a significant threat to human health. Therefore, new treatment options, including alternative antimicrobial agents, are being sought. Taking into account that the combination of bacteriophages with antibacterial metal ions could provide a synergistic effect, as well as the extensive use of hydroxyapatite (HAp) in the development of bone implant materials, Zn-substituted HAp (ZnHAp) nanoparticles’ interaction with bacteriophages was evaluated for the first time. The physicochemical and antibacterial properties of the ZnHAp nanoparticles were characterized. Pure-phase HAp nanoparticles with Zn content up to 0.74 ± 0.07 wt% were synthesized using wet chemical precipitation. The ZnHAp nanoparticles did not show antibacterial activity against Gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria. In turn, the ZnHAp nanoparticles’ antibacterial activity against Gram-positive Staphylococcus aureus (S. aureus) bacteria depended on the Zn concentration and the incubation time - the highest efficiency was shown by the HAp nanoparticles with 0.74 ± 0.07 wt% of Zn after 72 h. P. aeruginosa and S. aureus bacteriophages retained lytic activity after incubation in the presence of the nanoparticles, showing activity against host bacteria, i.e., a moderate titer (106-108 PFU/mL), yet corresponding to an effective therapeutic titer both in the bacteriophage suspension and sediments after decantation. The current findings illustrated that combining lytic phages with metallic ions substituted HAp nanoparticles is a promising technique for obtaining novel biomaterials with the prevention and treatment potential of polymicrobial and biofilm infections.


Atslēgas vārdi
Hydroxyapatite; Zinc ions; Bacteriophages; Antibacterial properties
DOI
10.1016/j.colsurfa.2025.137114
Hipersaite
https://www.sciencedirect.com/science/article/pii/S0927775725010179?via%3Dihub

Rezevska, D., Stīpniece, L., Rubene, L., Ščeglova, M., Mauriņš, S., Vasiļjevs, R., Rācenis, K., Kroiča, J. Zinc-Substituted Hydroxyapatite-Bacteriophage Complexes for Complementary Antibacterial Properties. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2025, Vol. 720, Article number 137114. ISSN 0927-7757. e-ISSN 1873-4359. Pieejams: doi:10.1016/j.colsurfa.2025.137114

Publikācijas valoda
English (en)
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