The research is dedicated to microclimate regulatory clothes with an integrated electrical system, which includes Peltier elements, and cooling function is based on the heat conductivity in solids. Several experiments have demonstrated the possibility of system operation. To verify microclimate regulatory vests compliance with the general requirements to the garment, it is scheduled to assess the physiologically - hygienic point of wearing by the appropriate operating conditions, during activities by measuring both temperature changes in different locations in space between the body and clothes and other physiological parameters (resistance, humidity etc.). Temperature measurements modeling experiments are performed in the space between the garment first layer and cotton T-shirt, to determine changes of the body microclimate at rest and during the time of physical activities (11 points: area of system operation and control area): 1) measurements with switched off cooling system; 2) measurements with switched on cooling system. The finding results demonstrate the feasibility of the system’s operation, the external environmental temperature influence on the measurement results, as well as the negative impact of the cooling system on the sweat vapor removal efficiency.