The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg
IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics
2013
Kristīne Veģere,
Ieva Bērza,
Simona Larsona,
Andris Dišlers,
Juris Vanags
Biotechnological aspects in the field of recombinant
protein production are analysed in this work, which includes
the investigation of factors for the production of virus
like particles (VLPs) formed by recombinant Hepatitis B virus
core-antigen (HBcAg) in inducible bacterial (E. coli) expression
system as well as purification of these particles by gelfiltration
and ion-exchange chromatography. Biomass yield
and VLP production level depended on the cultivation conditions
such carbon source and temperature. As inductor for
protein expression lactose was used.
Keywords
Recombinant Hepatitis B core-antigen, virus like particles, T5 promoter, expression
DOI
10.1007/978-3-642-34197-7_28
Hyperlink
http://link.springer.com/chapter/10.1007/978-3-642-34197-7_28
Ruģele, K., Bērza, I., Larsona, S., Dišlers, A., Vanags, J. The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg. In: IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics, Latvia, Rīga, 10-12 October, 2012. Berlin: Springer Berlin Heidelberg, 2013, pp.108-111. ISBN 978-3-642-34196-0. e-ISBN 978-3-642-34197-7. ISSN 1680-0737. e-ISSN 1433-9277. Available from: doi:10.1007/978-3-642-34197-7_28
Publication language
English (en)