Derivatization of glycerol and its formates with 4-nitrophenyl isocyanate
Chemical interesterification reaction of rapeseed oil with methyl formate yield two layers of product - upper biofuel containing layer and lower glycerol formates containing layer. Our aim was to develop a method for analysis of lower layer of interesterification product containing glycerol, monoformin (MFG), diformin (DFG), and triformin (TFG) in lower layer of interesterification reaction. Underivatized glycerol and MFG cannot be determined with HPLC-PDA-MS. Derivatization was performed with 4-nitrophenyl isocianate at first attempt. Reagent does not dissolve completely and the products also are sparcely soluble in acetonitrile. N,N-dimethylpyridine was tested as catalyst. Reaction products and partially derivatized intermediates were identified. We concluded that this type of derivatization probably could perform better, when using phenyl isocyanate.
Pieteikuma datums
28.01.2019.
Atslēgas vārdi
derivatization, 4-nitrophenyl isocyanate, glycerol
