Scaffolds used for bone tissue regeneration should provide void volume for new tissue formation and vascularisation, ensure cell attachment/proliferation and they must show suitable mechanical properties in order to repair non-healing or critical size bone defects. Thus, an ideal scaffold should be three-dimensional and highly porous with an interconnected pore network. One of materials that can be used for highly porous scaffold production is TiO2 ceramic due to its excellent mechanical properties compared to other ceramic materials, biocompatibility and good osteoconductivity. The aim of present research was focused on the preparation and modification of novel tissue engineering scaffolds with suitable mechanical properties and favourable microstructure based on porous TiO2 ceramic. Porous TiO2 scaffolds were produced via polymer foam replica method. The impact of TiO2 slurry processing parameters and thermal treatment on physical properties of obtained scaffolds was evaluated. Using replica method porous TiO2 ceramic with porosity >85% and pore size >100 μm were obtained. Scaffolds showed fully open and interconnected pore structure.