The development of cost-efficient, highly productive technologies for fermentation feed production from lignocellulose biomass is still a challenge. In this paper, the production of fermentable sugars from lignocellulosic biomass using hydrolysis techniques with membrane separation systems is studied. The research was conducted on both a laboratory and pilot level to evaluate and optimize the efficiency of the proposed technology. The results demonstrated that UF and NF permeate recovery increased efficiency, and the highest sugar recovery rates were obtained when secondary waste recirculation was introduced after NF and UF, reaching an almost 40% yield from all produced sugars.