Lignocellulose-degrading fungi are considered as the most promising strategy for biomass utilization. In this work two different fungal isolates were investigated for their ability to produce lignocellulose-degrading enzyme during cultivation process using hay substrate as a carbon source. The extracted enzymes were compared with commercially available cellulotic enzyme mixture Viscozyme L (Sigma-Aldrich). Filter paper units (FPU ml-1) were determined. The results demonstrated that I. lacteus produced a lignocellulose-degrading enzyme with an activity of 1.195 U ml-1 and was able to release almost 30% from available reducing sugars while no enzyme activity was detected from F. solani.