In today’s modern world there still are large number of areas around the globe, where infections and diseases are caused by untreated drinking water. Usually, such problems occur in poor and developing countries or rural areas, where centralized treated tap water supply is not available. One of the approaches for water treatment is membrane ultrafiltration (UF) that can filter most of microorganisms, but unlike reverse osmosis still preserve valuable minerals in drinking water. UF has several advantages over other filtration and pathogen reducing technologies, i.e., low energy consumption, low cost, compactness and possibility of installation on existing water supply infrastructure. The objective of the present study was to investigate the efficacy of PES C10 PDADMAC Layer-by-Layer coated ultrafiltration membrane to remove microbial pollution.