Fibrin is a blood component involved in the clotting process and can be used as a matrix for tissue engineering applications [1]. Vancomycin, a glycopeptide bactericidal antibiotic, has been used in human medicine for over 40 years [2]. The aim of this study was to develop autologous fibrin matrices for controlled vancomycin hydrochloride (V-HCl) delivery and determine their antibacterial properties against Staphylococcus aureus (ATCC 25923). Samples were prepared from donor blood and V-HCl was encapsulated in microcapsules for ensuring the sustained release kinetics.