Bone grafting is the second most commonly used surgical procedure in clinic right after blood transfusion1. Traditional treatment for bone regeneration such as autografts has been widely used, but it brings some serious disadvantages, such as infection, pain and the need for additional surgery. Thus, there is a critical need for new therapeutic strategies to repair bone damage caused by traumatic injury or osteoporotic fracture. An ideal bone grafting material should be bioactive, injectable, antibacterial, provides appropriate mechanical properties and act as a drug delivery system2. The aim of this study was to develop and investigate bioactive composite hydrogels based on natural biopolymers - hyaluronic acid (HA) and ɛ-polylysine (ɛ-PL), as well as strontium ranelate loaded nanosized hydroxyapatite (SrRAN-nHAp).