The increasing concerns of food packaging waste on the environment are raising interest on more sustainable polymer materials and their composites to reduce environmental impact. In this case bio-based polymers are considered as a less harmful alternative to traditional plastics [1]. To enhance the properties of bio-based polymer composites, additional natural fillers were chosen. The nanoclay filler was used as to improve the polymer matrix mechanical and thermal properties [2]. Additionally, to improve antibacterial properties chitosan with different degree of acetylation was used [3]. Bio-based polyester from NaturePlast with either 35% (PBE 001) or 50% (PBE 003) of biobased polymer content was used as a matrix for packaging films. The bio-based polymer composite samples with nanoclay (Nanocor) at 1 – 5 wt.% and chitosan (C95 and C98) at 3 – 10 wt.% were prepared by laboratory two-roll mills. Samples for rheology, dynamic mechanical analysis and tear resistance tests were obtained by compression molding. Results showed that C95 and C98 chitosan fillers from 3 to 10 wt. % increase melt viscosity of the bio-polymer matrix whereas the nanoclay effect on the viscosity increase was to a lesser extent. In contrast dynamic mechanical analysis showed that nanoclay filler at higher concentrations improved bio-based polymer composite storage modulus indicating on reinforcing effect while chitosan effect on this parameter was minimal. Similar reinforcing effect of the nanoclay was observed in tear test of the bio-based polymer films