The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg

IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics 2013
Kristīne Veģere, Ieva Bērza, Simona Larsona, Andris Dišlers, Juris Vanags

Biotechnological aspects in the field of recombinant protein production are analysed in this work, which includes the investigation of factors for the production of virus like particles (VLPs) formed by recombinant Hepatitis B virus core-antigen (HBcAg) in inducible bacterial (E. coli) expression system as well as purification of these particles by gelfiltration and ion-exchange chromatography. Biomass yield and VLP production level depended on the cultivation conditions such carbon source and temperature. As inductor for protein expression lactose was used.

Atslēgas vārdi
Recombinant Hepatitis B core-antigen, virus like particles, T5 promoter, expression
DOI
10.1007/978-3-642-34197-7_28
Hipersaite
http://link.springer.com/chapter/10.1007/978-3-642-34197-7_28

Ruģele, K., Bērza, I., Larsona, S., Dišlers, A., Vanags, J. The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg. No: IFMBE Proceedings. Vol.38: International Symposium on Biomedical Engineering and Medical Physics, Latvija, Rīga, 10.-12. oktobris, 2012. Berlin: Springer Berlin Heidelberg, 2013, 108.-111.lpp. ISBN 978-3-642-34196-0. e-ISBN 978-3-642-34197-7. ISSN 1680-0737. e-ISSN 1433-9277. Pieejams: doi:10.1007/978-3-642-34197-7_28

Publikācijas valoda
English (en)