Azolylpurine nucleoside derivatives exhibit a broad range of notable biological activities. Our group has recently investigated regioselective SNAr reactions of 2,6-bis-triazolyl purine nucleosides with primary and secondary amines and thiols. The obtained N6-substituted 2-triazolyl adenosine analogs possessed good fluorescent properties. Herein, we report the synthesis of purine nucleoside – amino acid conjugates. The obtained nucleosides showed useful levels of fluorescence with quantum yields up to 38% and Stoke shifts up to 91 nm. The chemoselectivity of nucleophilic aromatic substitution between bis-triazolylderivative and amino acids containing both N- and S-nucleophilic sites are discussed.