Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A3/A4 Conjugates

Antibiotics 2017
Andris Zeltiņš, Māris Turks, Dace Skrastiņa, Jevgeņija Lugiņina, Ieva Kalnciema, Ina Balke, Ērika Bizdēna, Vitālijs Skrīvelis

Milbemycins are macrolide antibiotics with a broad spectrum of nematocidal, insecticidal, and acaricidal activity. To obtain milbemycin A 3 /A 4 derivatives suitable for chemical conjugation to protein carriers (milbemycin haptens), succinate linker and a novel 17-atom-long linker containing a terminal carboxylic acid group were attached to the milbemycin core in a protecting group-free synthesis. The obtainedmilbemycin A 3 /A 4 derivativeswere coupled to Potato virus Y-like nanoparticles by the activated estermethod. The reaction products were characterized and used inmice immunization experiments. It was found that the mice developed weak specific immune responses toward all tested milbemycin haptens.

Atslēgas vārdi
ELISA | Hydrophobic hapten | Macrocyclic lactone | Plant virus
DOI
10.3390/antibiotics6030018
Hipersaite
http://www.mdpi.com/2079-6382/6/3/18

Zeltiņš, A., Turks, M., Skrastiņa, D., Lugiņina, J., Kalnciema, I., Balke, I., Bizdēna, Ē., Skrīvelis, V. Synthesis and Immunological Evaluation of Virus-Like Particle-Milbemycin A3/A4 Conjugates. Antibiotics, 2017, Vol.6, Iss.3, Article number 18. ISSN 2079-6382. Pieejams: doi:10.3390/antibiotics6030018

Publikācijas valoda
English (en)